Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Spectramax id3 multimode microplate reader molecular. Spectramax m3, m4, m5, and m5e multimode microplate reader. I used spectramax m3 plate reader to measure the fluorescence change through time. Softmax pro software has been integrated by many leading robotics and. All spectramax m series multimode microplate readers measure uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The spectramax m3, m4, m5, and m5 e microplate readers are a series of dualmonochromator, multidetection, multimode instruments with a triplemode cuvette port and 6well to 384well microplate reading capability. Spectramax m3 multimode microplate reader, see page 202. Now you can run both standard spectrophotometer and microplate reader applications on the same instrument. Softmax pro software user guide marine biological laboratory. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectramax m2 readers provide for easy conversion and optimization of verylowthroughput to mediumhighthroughput assays, faster, more precise results and reagent savings.
Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. Explore more about the spectramax i3x multimode plate reader, a userupgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Spectramax m3 laboratory equipment pdf manual download. Spectramax plus384 is the only spectrophotometer with a builtin cuvette port and microplate drawer.
The spectramax m3, m4, m5, and m5 e microplate readers are a series of dualmonochromator, multidetection, multimode instruments with a triplemode cuvette port and 6well to 384well microplate reading. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence. The optical performance is comparable to a topoftheline dedicated. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection. Spectramax microplate spectrophotometer operators manual. Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16. The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a. Combine pdfs in the order you want with the easiest pdf merger available. The spectramax m2 and m2e readers provide two detection modes in one platform, and offer the the added functionality of bottomread fluorescence for cellbased assays m2e.
Choose from a three m3, four m4, or five m5m5e mode reader. Dec 03, 20 the spectramax minimax 300 imaging cytometer enables cell visualization and cellbased analysis on the spectramax i3 multimode microplate reader. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Accumulated intermediates, in the presence of reduced glutathione, are transported to the vacuoles, whereupon the development of the red color. Molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific.
The spectramax m5 multimode microplate reader is owned by the nadler lab and available to all investigators through user fees this reader does uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes, standard. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. One of our customer services cs requirement was to be able to retrieve from m3 a combined. The colorimetric peroxidase substrate tetramethylbenzidine was quantified by measurement of absorbance at 450 and 540 nm spectramax m3, molecular devices. Spectramax m3 multimode microplate reader, molecular devices. The cuvette chamber spectramax plus 384 only figure 1. You can also use merge to combine an entire scene with another. This featurerich reader has a large, highresolution touchscreen with an embedded software package allowing you to quickly set up and run custom protocols.
Group sections enable you to combine the wells in a plate or several cuvettes into groups. Spectramax i3 spectratest fl1 protocol softmax pro. Spectramax m3, m4, m5, and m5 e multimode microplate readers. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. Type the name of the file to which you want to append this file. The spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes.
Save as pdf to save the printed output of a file to a portable document format. Microplate readers, multimode, spectramax m series vwr. Endpoint, kinetic, spectrum, and multi point well scanning applications combining. Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a. Spectramax plus 384 microplate reader molecular devices. Spectramax m2m2e user guide university of michigan. Accumulated intermediates, in the presence of reduced glutathione, are transported to the vacuoles, whereupon the development of the. Merge allows you to load objects from saved scene files into the current scene. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects. This document is in pdf format and can be viewed using a pdf viewer like adobe. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. For compounds with a known extinction coefficient, concentration can be calculated directly from absorbance. Ten to thousand time diluted samples were used in the assay according to the manufacturers instructions.
Oral administration of heattreated lactobacilli modifies. This revolution in modularity makes nocompromise detection the norm. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Molecular devices spectramax m3 multi mode microplate reader. Flexible throughput read one sample or up to 384 at a time its your choice. Spectramax m series multimode microplate readers 7 the spectradrop microvolume microplate offers the ability to use as little as 2l samples with 24 or 64well plates.
Spectramax id3 and id5 multimode microplate readers. Molecular devices spectramax id3 multimode microplate reader. Choose from a three m3, four m4, or five m5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities. Every tool you need to use pdfs, at your fingertips. The spectramax m3, m4, m5 and m5e are modular, upgradeable dualmonochromator microplate reader platforms that allow you to upgrade with other detection modes at a later time. Uvvisible absorbance abs fluorescence intensity fi glow luminescence lumi the. With optimized reagents and the industryleading data acquisition and analysis tool softmax pro 7 software, the spectramax id3 allows customization of workflow to address challenging research applications. Merge, split, compress, convert, rotate, unlock and watermark pdfs with just a few clicks. Spectramax m series multimode microplate readers biothing. The broad array of spectramax i3x detection cartridges enables the user. Luciferase detection instrumentation guide thermo fisher.
Acinterlab provides quality, brandname equipment, reagents and supplies to laboratories across the world. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Spectramax m3 multimode microplate reader, see page 122. With optimized reagents, option to operate the readers.
All m series readers accept cuvettes and 6, 12, 24, 48, 96, and 384well microplates. Spectramax plus 384 microplate reader a microplate spectrophotometer with a builtin cuvette port the pathcheck sensor also allows for the elimination of standard curves. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Spectramax plate reader eastern virginia medical school. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. The following document is intended to demonstrate setup of this instrument. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. Spectramax i3 spectratest lm1 protocol softmax pro. Separate one page or a whole set for easy conversion into independent pdf files. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters.
In this manual, we use the terms absorbance and optical density. We therefore gathered salient information about many of the instruments available to date. Life sciencemolecular devices spectramax 384plus from delhi. The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. The spectramax m3, m4, m5, and m5e microplate readers are a series. Spectramax m3, m4, m5, and m5e multimode microplate. Molecular devices spectramax id3 multimode microplate. Spectramax plus 384 microplate reader manual conquer. Compatible automation solutions for spectramax readers.
Turn on the plate reader with the rocking toggle switch on the back righthand side. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. Spectramax plus 384 microplate spectrophotometer operator s manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Insert your plate into the blue adapter, making sure it is lying flat inside the clips. Spectramax m series multimode microplate readers molecular.
I wonder if anyone knows how i can combine these values and make it into one. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Superior flexibility the spectramax i3 multimode platform comes with standard spectral absorbance, fluorescence, and luminescence detection.
The spectramax m series multimode microplate readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Apr 07, 2015 molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific. Spectramax m series multimode microplate readers from. Spectramax m3 multimode microplate reader from molecular. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. Microplate readers laboratory equipment laboratory. These settings are also valid for the spectramax m3m4, flexstation 3 multimode. Molecular devices versamax microplate reader the molecular devices versamax elisa microplate reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequencefree assay flexibility. Setup guide on the molecular devices spectramax m5m5e. M5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities. Softmax pro data acquisition and analysis software.
Spectramax m series molecular devices pdf catalogs. Molecular devices versamax microplate reader marshall. Key features upgradeable platform for changing lab needs threemode cuvette port for assay development. Spectramax m3 microplate reader from molecular devices. Spectramax i3 multimode detection platform youtube. The versamax microplate reader is built around a grating monochromator. Molecular devices just launched spectramax id3 multimode microplate reader is the cornerstone of complete laboratory solution helping to expand the boundaries of research. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform.
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